This study evaluates the knowledge, acceptability, perceptions of barriers, and cost of HPV infection and vaccination among unvaccinated Indian adults in the context of rising health concerns related to HPV and historically low vaccine uptake. It used a quantitative crosssectional study design, and thirty-three closed-ended questions from a structured online survey were used to collect data. Research participants were recruited between March and early May 2025 from urban, suburban, and rural settings in India and were adults, aged 18 and older. Descriptive statistics and chi-square tests p<0.05 were run on a final sample of 248 eligible responses to examine associations between demographics and knowledge or acceptance levels. Results revealed that 30.65% of participants were aware of how HPV is spread, 73.39% had heard of the HPV vaccine, and 6% were aware of the screening process. About 51.6% of respondents had moderate or low knowledge of prevention, compared to 48.4% who had high knowledge. The research revealed no significant statistical relationships yet female survey participants from cities with higher earnings and better education outcomes tended to know more about these vaccinations. Most common barriers faced by men against vaccination was because they encountered cost, lagging knowledge as well as insufficient healthcare guidance and targeted stigma. In addition, while 83% identified cervical cancer as a public health problem, the nonavalent vaccine generated low willingness to pay as financial limitations were a major barrier. No significant relationship was found between the level of Knowledge and the age, gender or residence of the population according to Chi-Square tests, however relationship was found between age and acceptability levels. The findings confirm that awareness around the risks associated with HPV still leaves a gap in knowledge and presents socio- cultural barriers. High acceptability rates stress the importance of better education together with healthcare provider engagement and government-supported vaccine subsidies to boost vaccination rates. In India the burden of HPV disease can be significantly reduced by incorporation of HPV vaccination into the national immunization program, laying emphasis on gender-neutral vaccination policies, and focusing on removing myths and misconceptions about the vaccine. Overall, this study provides important information to those invested in increasing adult vaccination rates in a public health approach that gives people information, access, and affordability