The increasing prevalence of biologic and biosimilar medicines in healthcare has necessitated a comprehensive understanding between medical professionals, comprising community pharmacists, who have a vital part in medication management and patient education. This study aimed to assess the community pharmacists in Dubai’s perspectives and knowledge of the usage of biologics and biosimilar medications. A cross-sectional study was carried out with community pharmacists practicing in various community pharmacies across Dubai, to evaluate their knowledge, perceptions, and confidence levels related to these therapies. The study offers strategies and approaches to raise awareness and knowledge regarding Biosimilars. Using a questionnaire created in Microsoft Forms, a quantitative survey-based methodology was used to conduct the study. A link to the survey was sent to community pharmacists in Dubai. 148 healthcare professionals responded to the survey, among which 111 eligible respondents were finalized. The data was analyzed statistically using Excel and Python software. The findings revealed varying levels of awareness, with a majority of pharmacists demonstrating a basic understanding of biologics and biosimilars but expressing a need for more in-depth knowledge and training. Perceptions regarding the safety, effectiveness, and substitutability of biosimilars were generally mixed, and around 45% of respondents, responded that they felt ‘somewhat confident’ about their ability to distinguish between biologic and biosimilar drugs. The study identified the major barrier and facilitating factor in biosimilar adoption as concerns about safety and efficacy and better education and training respectively. The most strongly suggested recommendation to the healthcare authority is to implement educational initiatives with 56% of response. Comprehensive continuing education courses is clearly the most prioritized strategy to prevent misconceptions and knowledge gaps among pharmacists with 52% of respondents chose it as their first choice. The statistical analysis there is significant correlation between participation in training or continuing education on biologics and biosimilars with higher levels of knowledge among pharmacists which is proved the p value of -1.204. The study highlights the need for targeted educational initiatives to enhance pharmacist’s competencies in this rapidly evolving area, ensuring they are well-equipped to support optimal patient care and contribute to the safe integration of biologic and biosimilar medicines in clinical practice.